e1l3n  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Cellular and molecular profiling of collagenous gastritis implicates pathogenic CD4 + T cells

doi: 10.1016/j.isci.2025.114344

Figure Lengend Snippet: Patients with Collagenous gastritis have an influx of highly activated T cells (A) CD4 + and CD8 + T cells were quantified by flow cytometry of the duodenum (top row) or stomach (bottom row). (B) Representative flow plots from (A). (C) CD4:CD8 ratio from (A). (D and E) PD-1 expression on duodenal CD8 + (D) or CD4 + T cells (E). (F and G) PD-1 expression on gastric CD8 + (F) or CD4 + T cells (G). (H) Representative CD8 and CD4 stains from tissue sections. All images at 20x objective. Scale bars 500 μm. (I) Quantification of (H) for intraepithelial (top row) or intraepithelial and stromal lymphocytes (bottom) from slides. Total cells per five high power fields (HPFs). (J) Quantification of PD-L1 and PD-1 stained tissue sections. (K) CD4: CD8 ratio from T cells captured by scRNA-seq (see D) in CD45-sorted samples. Significance assessed by the Wilcoxon rank test. (L) Heatmap of differential gene expression comparing control patients to each CG patient sample for CD8 and CD4 scRNA-seq clusters from CD45+ sorted cells. Genes involved in T cell activation/exhaustion are shown. Dot indicates significantly different expression between a patient sample and all control controls. Data are mean ± SEM; flow percentages were compared with a Student’s t test (flow) or Wilcoxon test (histology) followed by a Benjamini-Hochberg correction. Gene expression data compared with a Benjamini-Hochberg-corrected Wilcoxon rank-sum test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 See also and .

Article Snippet: PD-L1 (E1L3N®) XP® Rabbit mAb , Cell Signaling Technology , #13684S; RRID: AB_2687655.

Techniques: Flow Cytometry, Expressing, Staining, Gene Expression, Control, Activation Assay